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Agilent Chemstation Software installation with instrument hardware and compatibility considerations.

Introduction Agilent OpenLAB is a portfolio of laboratory software, which provides an open architecture and reusable, standardized interfaces. There are different OpenLAB solutions for each step in the life cycle of scientific data: †Chromatographic Data System (CDS) OpenLAB CDS is available as EZChrom Edition or as ChemStation Edition.

Overview

Agilent is a company that spun off from Hewlet Packard (HP) to produce scientific instrumentation which includes Liquid Chromatography (LC), Gas Chromatography (GC), and Mass Spectrometry (MS) equipment. Much of the earlier network hardware such as the network cards are adapted network printer cards. Agilent have taken the approach that one software package called Chemstation will co-ordinate and drive all their equipment.

1. Agilent Chemstation Software Manual 5,7/10 5428 reviews. 1.0 OBJECTIVE: To lay down the procedure for operation and calibration of High Performance Liquid.
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3. Here is a 'best of' collection of links to manuals and tools for OpenLab CDS on Agilent.com, for Chemstation edition, see Collection of OpenLab CDS ChemStation Resources. For other software and instruments, refer to Collection of Support Resources.
4. View & download of more than 3203 Agilent Technologies PDF user manuals, service manuals, operating guides. Measuring Instruments, Test Equipment user manuals, operating guides & specifications.

Chemstation Software

Chemstation software comes with each complete purchase of a LC, GC or MS machine. Over the years newer versions of Chemstation have been released which differ in there compatibility with different versions of Windows starting from windows NT 4. A license code is provided which unlocks the appropriate part of the Chemstation software so it can be run with the hardware sold.

Chemstation also relies on the Microsfot .net framework and MS redistributable 2005 c++ library to run and communicate with the hardware modules in each instrument. Sometimes the modules are all integrated into a single instrument like an all-in-one stereo unit. Other times the modules are stackable and interchangeable like a stereo with separate CD, radio, amplifier etc.

The Hardware

The computer and Chemstation compatibility.

Chemstation software will run on most i486 or higher computers. Newer versions of Chemstation may not install on older versions of Windows. XP versions will not run on Windows 2000 profession (NT4) for example. Older versions of Chemstation may install on newer Windows versions but with limitations. Because of the requirement for specific .net versions for example which may not be compatible with newer Windows versions analysis of data may be done but the hardware control will fail. There is a workaround to allow 32 bit Chemstation to operate on 64 bit computers to allow analysis but not control of the hardware. Essentially you point the .net framework in a Windows installation from the default 64 bit version of .net to a 32 bit version during install of the software. It can be pointed back to the 64 bit version of .net after the install if required.

The instructions are:

Modules

Stackable module All-in-one.

There are dozens of different possible hardware modules either built into a single instrument or as stackable units or modules. They typically have names that include functions such as pump, reader, plate, analyser, column etc. From an IT perspective they are all the same. Each module contains a version of firmware that identifies it to other instruments and computers it is plugged in to. Often the module has the type of firmware on a sticker at the front of the unit such as G1234B. Some modules will only run when plugged into another module. Adobe photoshop dmg crack mac. Such “dumb” modules have no firmware to communicate their existence. Modules can communicate via:

1. Local Area Network (LAN) setups.

2. GBIB interface. Detail of this type is left out here as only very old machines still use these.

3. CAN interface. The CAN cables look like ethernet cables but are not the same and can also provide power. Typically they have metal (earthing?) around the plugs at each end.

4. Serial interface used with the “dumb” modules.

Typically only one module in a single setup is required to have a LAN or GBIB interface. Usually more than one module is present and the modules are typically connected via the CAN cables to each other in a serial fashion. One module in any one setup will have a LAN or GBIB card in it to allow connection of the rest of the modules to a computer if the interface card has been purchased and installed in the “master” module. If no networking interface is installed the modules are controlled by a special controller with keypad which plugs in to a CAN interface on one of the modules. All-in-one units will have the special controller built in.

Example of separate controller for modular system. All in one systems have a keypad and screen built in.

Setting up an Agilent system with a computer running Chemstation.

1. Ensure you have a version of Chemstation to install which has a license for the type of machine/modules you wish to use. A license for a Gas Chromatography setup will not allow you to run a Liquid Chromatography machine even though the same CD is used to install the software. DO not install the software at this stage.

2. Ensure the MS Windows version is appropriate for the age of the Chemstation software. Chemstation also sell separate 32 bit and 64 bit versions. As indicated earlier 32 bit versions may install and run on 64 bit windows for analysis only using the right “tweak” but will not control the instruments.

3. Ensure the firmware version in each module is appropriate for the version of Chemstation. As of August 2013 all current versions of firmware were reverse compatable with older Chemstation software. It is best to simply download the current versions of the firmware from the Agilent web site and update all the modules to the latest version if they are not already up to date. A link cannot be given here as Agilent require each user to register before downloading the firmware updates so each person gets a unique URL to download from. There is a special tool which is also downloaded from the site or is available off the Chemstation install CD called something like FWudate.exe to allow you to install the new firmware.

4. Plug any modules together with the CAN and serial cables as appropriate. Ensure you use an ethernet cable for the connection from the LAN card installed in the master module to the computer ethernet port. If the master module or all-in-one has a GBIB card instead you will need a GBIB cable and a GBIB card in the computer.

5. Set the IP address on any modules that have network capability. BOOTP is suggested in the manuals but it is best to avoid this. In the current (2013) university network environments a bootp server may “go wild” if accidently plugged into a university network port and disrupt communications for everyone. The default IP address for a module’s network card may be something like eg. 192.168.254.11. Older and perhaps newer modules use HP printer type network hardware which allows HP JetDirect software to interrogate the card. All-in-one machines will have an interface built in which allows you to look at the IP addresses. Modules will require a special LCD hand held controller that plugs into a CAN port to view and change settings. Setting the computers network card to the appropriate subnet and pinging the module’s network IP address from the computer will confirm communication. At least in one case the default IP address for the network card did not “stick” and would reply to pings under some circumstances but not others as its address would change to 000.000.000.000 . This can make setups almost impossible. It is important to make sure communication is reliable before proceeding past this step. Occasionally the module containing the network interface may need rebooting. It pays to make sure it is manually set to the default address using the inbuilt screen on all-in-ones or the plug in controller for the modular setups.

6. Set the computer’s network card to the appropriate subnet and IP address eg. 192.168.254.1 . Do a ping to ensure the instrument is listening. A fresh boot of the instrument may be needed. Use the firmware update software on the install CD of off the Agilent website to check the status of the modules in the system. It is called something like fwUPdate.exe. The date of the last module update should be at least within a few years of the time of the version of the Chemstation install. If there is doubt use the update software to update the modules to the latest version. As of August 2013 Agilent were ensuring the newest firmware could still be used by the older Chemstation versions.

7. Now that we have the platforms ready we can install the Chemstation software. During installation follow the prompts including adding the license key for the appropriate instrument type or types. You will be prompted for the “Instrument 1” setup. Edit Instrument 1’s configuration to reflect the machine type and IP address or GBIB configuration of the all-in-one or master module. Agilent I/O Libraries are a prerequisite for Chemstation. The newer versions of Chemstation will install this automatically when you run the installer. Older versions may require you to install the I/O Libraries manually.

8. All being well you should come to a screen which gives a list of potential modules to “add” to your current setup. If communication with the instruments is working well modules that are communicating their presence via the network interface will be green with the others grey. Add the green ones and finish the install.

1.0 OBJECTIVE: To lay down the procedure for operation and calibration of High Performance Liquid Chromatograph. 2.0 INSTRUMENT IDENTIFICATION: Name of Instrument: High Performance Liquid Chromatography (Auto Sampler) Manufacturer: AGILENT Technologies 1260 series 3.0 GENERAL CLEANING: 3.1 Ensure that the power supply to the instrument is switched off and main Cord is removed from supply.

3.2 Clean the instrument with a clean dry cloth every day. A wet cloth dipped In dilute soap solution may be used occasionally. 3.3 Precaution must be taken to clean the instrument immediately with dry Cloth after wet cleaning. 4.0 PROCEDURE (OPERATION) 4.1 Ensure that the HPLC system is placed in a clean and dust free area. 4.2 Ensure that instrument have been connected to the main power unit.

4.3 Switch ‘ON’ the Computer. 4.4 Switch ‘ON’ the HPLC instrument. (Switch on the detector, column compartment, Pump and vacuum degasser simultaneously). 4.5 Ensure that the outlet from the detector dips into a waste bottle.

5.0 Start the Open Lab Chemistration by double-clicking the Short cute Open Lab Chemistration located on your desktop. 5.1 Click on Open Lab Chemistration online. 5.2 Then click on ‘on’ option.

Agilent and the user have a separate. In this manual we describe the efficient use of the data acquisition, analysis. About the ChemStation Software 12.

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5.3 Initialization process of HPLC system will be start which shows by yellow colour. 5.4 Green colour and ‘Ready’ indication will be appearing on screen after the Initialization process. 5.5 Click on set up- pump, feed the flow rate NMT 4%,% of solvent and then start the purging by rotating the purge wall in anticlockwise direction approx 10 minute. 5.6 Then ‘OFF’ the Purging by rotating the purge valve in clockwise direction.

Make sure the sample to be run is properly loaded in the auto sampler your instrument. 5.7 Start an instrument application an Instrument Wizard will appear. This wizard is designed direct to the basic functions of the instrument.

5.8 Create or modify a method This button starts the Method Wizard that will enable you to step through creating or modifying a method. 5.9 When you start the Method Wizard, click to create a new method the wizard will Guide you through the method sections. For each section, enter the run Parameters Required for the sample you will be injecting 5.10 Data acquisition and instrument control parameters – (a) Instrument Setup. Select each tab and enter the correct parameters for running this sample (b) Integration events. Additional integration events are not necessary for a test Sample.

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Calibration of Peaks/Groups. Calibration parameters are not necessary for a test sample. From the File menu, select Report Template and then click Open.

From the list of templates, select Area%.rep to generate an Area%report when run the sample 5.11 Create a sequence This button starts the Sequence Wizard that steps you through creation of an acquisition reprocessing sequence. Or for creating sequence enter file menu which display Sequence, from Sequence enter new method. 5.12 Run one sample this button opens a dialog where you can use a stored method to run a single sample.

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• In the Single Run Acquisition dialog box, enter a number to be used as a Sample ID. • Click the method open button and then select the method file that was provided or the method you saved in the previous step. • Enter a Data file name you wish to use, or click the adjacent button to select a parameter that will be used to create the data file name for you. • Enter the Vial number and Injection Volume to be used.

5.13 Run sequence of samples this button opens the Run Sequence dialog where you can start data acquisition using a stored sequence. 5.14 Save the method. When you have completed setting up the method parameters, from the File menu, followed by Method and click Save As. Type a name to be used for your method and then click OK 5.15 Maintain Log book of HPLC 5.16 Report any discrepancy observed during operation and calibration of instrument to Section In charge or his representative for corrective and preventive action. 5.17 Section In charge or his representative will take the necessary action and report the same to Manager – Quality Control.